![]() ![]() ↳ Electrolyte Challenge: Orange Juice vs.↳ Do Oranges Lose or Gain Vitamin C After Being Picked?.↳ Math & Computer Science Sponsored by Hyperion Solutions Corp.↳ Grades 9-12: Getting Ready for the Science Fair.↳ Grades 6-8: Getting Ready for the Science Fair.↳ Grades K-5: Getting Ready for the Science Fair.↳ Science Teachers: Fairs, Projects, and General Support.↳ Advanced Science Competitions (Intel ISEF, Intel STS, Siemens Competition, JSHS, etc.).↳ Grades 9-12: Math and Computer Science.↳ Grades 9-12: Life, Earth, and Social Sciences.↳ Grades 6-8: Math and Computer Science.↳ Grades 6-8: Life, Earth, and Social Sciences.↳ Grades K-5: Math and Computer Science.↳ Grades K-5: Life, Earth, and Social Sciences.Active Forums (Make all new posts here).You have a complete project, but if you don’t obtain results, some creativity will be needed. If you cannot duplicate your experiment, and nothing grows, let me know and I will give you some advice for writing up your results. Using a culture that has grown up to late stationary phase will give consistent, reproducible results. If possible, I recommend growing up a broth culture overnight, making a 1:100 dilution in sterile culture medium, and transferring the diluted culture to a sterile Petri dish with agar to make the lawn and then place the antibiotic disks on the agar surface. Ideally, you want to make a “lawn” of bacteria from a freshly grown culture. The guidelines from the Science Buddies website contain helpful information for culturing bacteria and also techniques for troubleshooting: So continue to incubate your plates for a few more days. Here is a website that describes a typical bacterial growth curve this is very important background information for your project. Your older culture was probably in late stationary or early death phase, and if there are any survivors, it will take them time to start growing. Did you store your culture in the refrigerator? Did the culture dry out? Here is a website that describes the bacterial growth curve. If it has been 2 weeks since you received your bacteria, and you just transferred them to the agar plate, then they could still be recovering and in lag phase, and getting ready to grow. ![]() coli would grow up overnight and the zones of inhibition would be visible within 18-24 hours. How did you prepare the lawn of bacteria for the sensitivity test? What type of agar are you using? Is it possible that the surface of the agar has dried out? coli will grow very slowly at room temperature of 20-22 degrees C. Whatever temperature you use, this will be one of the controlled parameters in your experiment. If you don't find a warmer spot, then you will be using ambient temperature and it will just take a little longer for the bacteria to grow. If you are working with Petri dishes, avoid an area with too much air circulation, as you want to avoid drying out the plates before the colonies can grow. Make sure the temperature is not above 42 degrees Centigrade, as this could also kill the bacteria. As an alternative, you could put a light bulb in a cardboard box or check the temperature on top of your water heater. However, I don't recommend using the oven because a family member will invariably come in and turn the oven on without looking and destroy your project. If so, you can speed up their growth by incubating your samples in an enclosed container with a light bulb turned on, like your closed oven. Most likely you are working with mesophilic bacteria that grow well between ambient 20 and 37 degrees C. They are extremely simple to operate and make them the perfect choice for first-time chick incubators.What type of bacteria are you growing and what temperature do you want to achieve? Bacteria are very versatile and there are extreme psycryophiles that will grow at minus 15 degrees C and extreme thermophiles that grow at 80 degrees C. The best incubators for beginners certainly have to be ones with automatic turning abilities and automated settings. You can do this by arranging appropriate food, a heat lamp, a cage, and of course, making sure that you'll be able to appropriately care for the chicks once hatched. Once your eggs have been placed comfortably in the incubator, use the 21 day period to prepare for hatch day. Step 3 would be to set up your device correctly - ensuring that the temperature, humidity, and turning settings are appropriate. Get yourself an easy-to-operate incubator for classroom use. Secondly, you need the right tools to aid you in hatching the chicks. Remember that hatching chicks in an incubator takes a total of 21 days so firstly, be sure that you have the necessary time available and aren't starting the project before the school holidays begin! Hatching chicks in the classroom may sound like a difficult feat, but worry not! We've got 5 top tips to bear in mind when you want to incubate chicks at school.
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